In-vitro Culture of Latana camera from Nodal and Shoot-tip Explants in Phytoremediation Studies

This paper describes a prime and easy-touse protocol for large-scale production of plant lets through shoot tip culture of Lantana camera, the plant having phytoremediation potential and the method is useful for the ex-situ conservation of other species important for phytoremediation. A simple micro-propagation method from nodal and shoot tip explants were reported here for Lantana camera, at contaminated site nearby Bhopal. This plant is having higher phytoremediation potential. There are few research papers about the plant regeneration from nodal segments of Lantana camera. Thus it is necessary to work on in-vitro studies of Lantana camera. The plant regeneration from nodal segments were considered to be one of the most promising way for multiplying a selected variety. Here multiple shoots were induced in vitro from the stem nodal and shoot tip explants on Murashige and Skoog (MS) medium containing 6-benzylaminopurine (BAP), alone or with Naphthalene Acetic Acid (NAA) and kinetin (KN). Micro shoots were obtained in high frequency on MS medium having various concentrations of BAP (0.1 to 0.7 mg/l) along with NAA and KN from the explants. The shoot tip explants produced the highest number (3 per shoot) of shoots per culture with a average length of approximate 4.5 cm. The shoot tip explants produced maximum number of (1 to 2) shoots per culture on the same medium, average length of the in vitro shoots being approximate 4 cm. This tissue culture protocol helps to regenerate large number of Lantana camera plantlets having hyper accumulator properties for heavy metals. Keyword — Latana camera, MS Media, Shoot tip culture, Nodal explants, In-vitro, Phytoremediation.